Table 3.
In vitro contractile properties of soleus and extensor digitorum longus muscles of female skmERαWT and skmERαKO mice at 22 wk of age
skmERαWT | skmERαKO | P Value | |
---|---|---|---|
Soleus muscle | |||
n | 15 | 16 | |
Mass, mg | 9.9 (0.5) | 10.9 (0.4) | 0.114 |
Optimal muscle length, mm | 8.9 (0.2) | 9.3 (0.2) | 0.240 |
Physiological CSA, mm2 | 1.47 (0.066) | 1.56 (0.051) | 0.275 |
Peak twitch force, mN | 20.0 (0.9) | 21.6 (1.3) | 0.310 |
Twitch time to peak force, ms | 31.2 (0.7) | 34.2 (0.8) | 0.013 |
Twitch one-half relaxation time, ms | 39.5 (1.7) | 37.9 (1.7) | 0.563 |
Po, mN | 152.9 (5.0) | 157.7 (7.3) | 0.599 |
+dP/dt, N/s | 1.7 (0.05) | 1.8 (0.09) | 0.172 |
−dP/dt, N/s | 2.1 (0.07) | 2.3 (0.2) | 0.147 |
Active stiffness, N/m | 326.6 (10.9) | 324.6 (17.7) | 0.926 |
Peak concentric force, mN | 44.1 (2.8) | 40.7 (2.9) | 0.423 |
Peak eccentric force, mN | 292.0 (12.2) | 310.7 (13.1) | 0.309 |
Freq50, Hz | 21.4 (0.6) | 21.6 (1.0) | 0.921 |
Extensor digitorum longus muscle | |||
n | 13 | 15 | |
Mass, mg | 9.2 (0.2) | 9.1 (0.3) | 0.743 |
Optimal muscle length, mm | 10.5 (0.2) | 10.7 (0.3) | 0.688 |
Physiological CSA, mm2 | 1.87 (0.042) | 1.80 (0.058) | 0.582 |
Peak twitch force, mN | 87.2 (5.1) | 89.5 (5.0) | 0.766 |
Twitch time to peak force, ms | 20.4 (1.0) | 22.3 (0.4) | 0.182 |
Twitch one-half relaxation time, ms | 20.1 (1.2) | 24.6 (1.3) | 0.034 |
Po, mN | 319.7 (8.9) | 296.6 (11.9) | 0.091 |
+dP/dt, N/s | 9.0 (0.3) | 8.8 (0.4) | 0.675 |
−dP/dt, N/s | 15.2 (1.2) | 11.2 (1.04) | 0.024 |
Active stiffness, N/m | 614.0 (29.7) | 610.9 (34.1) | 0.947 |
Peak concentric force, mN | 130.7 (8.4) | 114.7 (11.1) | 0.057 |
Peak eccentric force, mN | 558.2 (19.5) | 478.9 (27.8) | 0.031 |
Freq50, Hz | 31.7 (2.0) | 26.1 (1.3) | 0.034 |
Values are means (SE). CSA, cross-sectional area; Po, maximal isometric tetanic force; +dP/dt, maximum rate of tetanic force development, −dP/dt, maximum rate of tetanic relaxation, Freq50, frequency at which 50% isometric tetanic force is generated.