Figure 2.

Reduced accumulation of macrophages in the peritoneal cavity of dmPGE₂-chimeric mice after injection of thioglycollate. Control-chimeric and dmPGE₂-chimeric mice were injected intraperitoneally with thioglycollate 4, 7, and 12 weeks after the chimeric mice were established. Cells were harvested from the peritoneal cavity after 3 days. (A) Total cells harvested from the peritoneal cavity of control-chimeric mice (solid line) and dmPGE₂-chimeric mice (broken line). The dotted horizontal shows the number of cells harvested from chimeric mice injected with an equal volume of saline. Data are shown as mean ± SEM (n = 6 mice/group). (B) Gating strategy [11] for identification of cell types (C, D) harvested from the peritoneal cavity 3 days after thioglycollate injection of 12-week-engrafted chimeric mice. (C) Number of total, resident, and elicited peritoneal macrophages. (D) Number of DCs, neutrophils (Neuts), and eosinophils. For (C) and (D), data are shown as mean ± SEM (n = 6 mice/group for control-chimeric mice; n = 4 mice/group for dmPGE₂-chimeric mice). Asterisk indicates a significant difference (p < 0.05) between cells harvested from control-chimeric and dmPGE₂-chimeric mice. ns = not significant.