Table 4.
Whole slide scanning analysis in VLM and medial raphe with mean values shown for all eight groups
| SST | NK1R | Galanin | TPH2 | SERT | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Group classification | VLM | VLM (smootheda) | VLM | VLM (smootheda) | VLM (smootheda) | MR (smootheda) | VLM | MR | VLM | MR |
| All SUDEP | 6.2 (1.4) | 2.4 (0.9) | 8 (1.7) | 1.5 (0.6) | 53.3 (18) | 54.4 (18) | 0.7 (0.7) | 1.7 (2) | 27.9 (14) | 40.4 (13) |
| n = 17 | n = 17 | n = 17 | n = 17 | n = 15 | n = 15 | n = 17 | n = 17 | n = 17 | n = 17 | |
| D-SUDEP | 6.3 (1.7) | 2.6 (1.2) | 8.2 (1.8) | 1.6 (0.8) | 48.6 (19) | 51 (20) | 0.5 (0.3) | 1.1 (0.9) | 34.9 (13) | 36.4 (9) |
| n = 10 | n = 10 | n = 10 | n = 10 | n = 10 | n = 10 | n = 10 | n = 10 | n = 10 | n = 10 | |
| SUDEP (excluding DS) | 6.2 (1.6) | 2.6 (1) | 7.7 (1.7) | 1.6 (0.7) | 49.7 (17) | 52.7 (19) | 0.7 (0.7) | 1.6 (1.7) | 38.1 (13) | 39.5 (11) |
| n = 14 | n = 14 | n = 14 | n = 14 | n = 12 | n = 12 | n = 14 | n = 14 | n = 14 | n = 14 | |
| Dravet syndrome | 6.1 (0.9) | 2.1 (0.4) | 8.9 (0.9) | 1.3 (0.4) | 60.6 (18) | 56.8 (17) | 1.3 (1.3) | 2.4 (3.7) | 41.2 (18) | 47.9 (16.7) |
| n = 7 | n = 7 | n = 5 | n = 5 | n = 6 | n = 6 | n = 7 | n = 7 | n = 7 | n = 7 | |
| EP-controls (excluding DS) | 5.2 (0.8) | 2.1 (0.5) | 7.7 (0.6) | 1.8 (0.4) | 53.2 (29) | 50.6 (26) | 1.8 (2.1) | 1.9 (1.0) | 40 (10) | 42.7 (10) |
| n = 6 | n = 6 | n = 6 | n = 6 | n = 6 | n = 6 | n = 6 | n = 6 | n = 6 | n = 6 | |
| All epilepsy controls | 5.6 (1.1) | 2.3 (0.5) | 7.9 (0.6) | 1.7 (0.4) | 56.5 (25.6) | 54.4 (23) | 1.5 (1.9) | 1.7 (0.9) | 42 (12) | 45.3 (10.8) |
| n = 8 | n = 8 | n = 8 | n = 8 | n = 8 | n = 8 | n = 8 | n = 8 | n = 8 | n = 8 | |
| NEC | 5.7 (0.9) | 2.7 (1) | 7.7 (2) | 2.2 (1) | 68.9 (9.5) | 67.3 (8.7) | 2.9 (3.4) | 5.7 (7.8) | 43.5 (20) | 52.8 (11) |
| n = 12 | n = 12 | n = 13 | n = 13 | n = 10 | n = 10 | n = 12 | n = 12 | n = 13 | N = 11 | |
| NESD | 5.5 (0.8) | 2.3 (0.3) | 7.2 (2) | 1.9 (0.5) | 67.4 (8.7) | 65.6 (7) | 2.0 (1.1) | 3.5 (2.1) | 42 (23) | 51.9 (12) |
| n = 10 | n = 10 | n = 10 | n = 10 | n = 9 | n = 9 | n = 9 | n = 9 | n = 10 | n = 8 | |
All obex levels are included in this analysis. All values are shown as labelling index [shown as percentage of area with immunostaining (i.e. range 0–100)].
n = the number of cases studied in each group with each marker (in occasional cases with each marker the section staining failed quality control).
a‘Smoothed’ data refers to additional Gaussian filters used on Definiens image analysis (see ‘Materials and methods’ section); for Galanin only the smoothed data is shown but both total and smoothed data showed significant differences between SUDEP and controls (see ‘Results’ section). Significant results highlighted in bold between SUDEP and controls (see ‘Results’ section). See also Supplementary Fig. 1 for graphs. Values in bold represent data with significant differences between SUDEP and control groups.
DS = Dravet syndrome; EP = epilepsy controls; MR = medial raphe; NEC = non-epilepsy controls; NESD = non-epilepsy sudden death controls.