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. 2018 Apr 10;33(6):1052–1062. doi: 10.1093/humrep/dey083

Figure 1.

Figure 1

LGR5 gene expression appears to decrease in the eutopic endometrium and Fallopian tube in the secretory phase of the menstrual cycle. (A) The eutopic endometrial samples and fallopian tube express apparently decreased levels of LGR5 mRNA in the secretory phase of the menstrual cycle when compared with the proliferative phase (n = 21). (B) Fallopian tube (at any stage of the cycle) demonstrate significantly higher levels of LGR5 mRNA than matched eutopic endometrium (P < 0.01) (n = 20). (C) Representative LGR5 ISH images of Fallopian tube and luminal, stratum functionalis and stratum basalis eutopic endometrium in the proliferative and secretory stages of the menstrual cycle (All images ×1000, scale bar = 20 μm, (n = 15)). (D) Graphical representation of semi-quantitative scoring of LGR5 ISH In the proliferative stage of the cycle, the luminal epithelium demonstrated significantly higher LGR5 ISH staining scores than the functionalis (P < 0.05), basalis epithelium (P < 0.01) and Fallopian tube (P < 0.02) as well as the luminal epithelium of the secretory phase (P < 0.03); the proliferative functionalis had significantly higher LGR5 scores than the secretory functionalis (P < 0.04); the secretory luminal epithelium showed significantly higher LGR5 scores than the epithelia of secretory stratum functionalis (P < 0.03), secretory stratum basalis (P < 0.05) and Fallopian tube (P < 0.02) (n = 7 per group) (Prol = Proliferative, Sec = Secretory Funct = stratum Functionalis, Bas = stratum Basalis).