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. 2018 Mar 1;69(12):2911–2922. doi: 10.1093/jxb/ery077

Fig. 5.

Fig. 5.

Overexpression of CER3 in the gcn5-2 mutant increases the cuticular wax component and restores it to the wild-type Ws level. (A) Relative expression levels of CER3 in plants of Ws, 35S::CER3/Ws (#2, #5, #10), gcn5-2, and 35S::CER3/gcn5-2 (#2, #5, #10). Total RNA was isolated from 6-week-old stems of Ws and the gcn5-2 mutant. ACTIN8 was used as an endogenous control. Data are means (±SD) from at least three biological replicates. (B) Scanning electron microscopy of the stems of Ws, 35S::CER3/Ws (#2, #5, #10), gcn5-2, and 35S::CER3/gcn5-2 (#2, #5, #10). Scale bars: 20 μm. (C) Total cuticular wax content was calculated over the unit area of 6-week-old stems in plants of Ws, 35S::CER3/Ws (#2, #5, #10), gcn5-2, and 35S::CER3/gcn5-2 (#2, #5, #10). The mean expression levels and total wax load were compared using one-way ANOVA together with a Bonferroni adjustment test in R. Different letters indicate significant differences among genotypes (P<0.05).