Fig. 2.

Effects of LasA on JA-regulated transcriptional activation. (A) Gene expression analysis of TAT3, JAZ1, JAZ5, and JAZ10 in wild-type (Col-0) plants in response to 50 µM LasA for 1 or 2 h; untreated plants (–) were included as controls. ACT8 was used as the housekeeping control gene. Statistically significant differences in gene expression between controls and LasA-treated plants were determined by two-tailed Student’s t-test (*** P<0.001). Each biological sample consisted of tissue pooled from 10–15 plants. Data are means (±SD) of three or four technical replicates. (B) GUS-staining for visualization of pJAZ2-GUS expression in roots of transgenic Arabidopsis in the wild-type and coi1-30 backgrounds. Seedlings (7-d-old) were treated with 5 µM JA or 100 µM LasA; untreated plants (–) were included as controls.