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. 2018 Mar 22;69(12):2869–2881. doi: 10.1093/jxb/ery112

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© The Author(s) 2018. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 4. — Loss of polar localization of PIN1:GFP in the stele cells of 3KO plants. PIN1:GFP was expressed in 3KO plants and its distribution in stele cells was compared with Columbia plants also expressing this reporter. (A) Columbia PIN1:GFP root tip; arrows mark two cells with typical polarized PIN1:GFP localization. (B) 3KO PIN1:GFP; arrows point to cells exemplifying depolarized PIN1:GFP localization. (C) Quantitative analysis of the proportion of cells (%) showing depolarized PIN1:GFP, evaluated by Scheffe analysis. Letters denote samples showing statistically insignificant (two ‘a’s) and statistically significant (‘a’ versus ‘b’ and ‘c’; ‘b’ versus ‘c’; P<0.05) differences. Scale bars=10 µm.