Table 2.
The impact of drought stress on transcription of selected genes (determined by RT-qPCR) in Arabidopsis thaliana wild-type (WT), 35S:CKX, DEX:CKX, DEX:IPT, SAG:IPT plants and Col-0 (WT) after meta-topolin treatment (Col-0 CK).
| HvCKX2 | IPT Agro | CKX1 | IPT3 | RD29B | P5CS1 | NCED3 | ||
|---|---|---|---|---|---|---|---|---|
| WT | BS | 1 | 1 | 1 | 1 | 1 | ||
| DC | 0.125 ± 0.074 | 0.129 ± 0.013 | 1.363 ± 0.430 | 0.130 ± 0.072 | 0.728 ± 0.081 | |||
| D | 0.916 ± 0.617 | 0.111 ± 0.058 | 8.390 ± 4.535 | 1.052 ± 0.171 | 5.690 ± 2.337 | |||
| RC | 0.637 ± 0.274 | 0.139 ± 0.054 | 5.029 ± 0.632 | 0.003 ± 0.001 | 7.378 ± 0.911 | |||
| R | 0.647 ± 0.327 | 0.305 ± 0.117 | 0.757 ± 0.128 | 0.357 ± 0.128 | 4.155 ± 1.182 | |||
| 35S:CKX | BS | 260.999 ± 95.301* | 1.478 ± 0.034* | 3.471 ± 0.414* | 1.380 ± 0.240* | 1.503 ± 0.246* | ||
| DC | NA | NA | NA | NA | NA | |||
| D | 168.162 ± 65.985* | 0.842 ± 0.097* | 1.225 ± 0.159* | 0.506 ± 0.107* | 3.936 ± 0.739 | |||
| RC | 121.291 ± 42.753* | 0.524 ± 9.7E-05* | 7.517 ± 1.879 | 1.001 ± 0.163* | 3.253 ± 0.281* | |||
| R | 0.334 ± 0.182 | 0.370 ± 6.8E-05 | ND | 0.499 ± 0.097 | 3.372 ± 0.318 | |||
| DEX:CKX | BS | 1.273 ± 0.449 | 0.932 ± 1.7E-04 | 0.843 ± 0.211 | 0.611 ± 0.099* | 1.576 ± 0.136* | ||
| DC | 1 | 0.192 ± 0.083 | 0.470 ± 0.122* | 0.443 ± 0.057* | 0.453 ± 0.082* | 1.697 ± 0.664* | ||
| D | 1.124 ± 0.280 | 0.413 ± 0.164 | 0.529 ± 0.081* | 1.808 ± 0.309* | 0.425 ± 0.070* | 5.668 ± 0.787* | ||
| RC | 0.303 ± 0.073 | 0.063 ± 0.022* | 0.068 ± 1.3E-05* | 0.047 ± 0.012* | 0.126 ± 0.020* | 0.463 ± 0.040* | ||
| R | 0.039 ± 0.018 | ND | ND | ND | 0.095 ± 0.125 | ND | ||
| DEX:IPT | BS | 1.410 ± 0.497 | 0.746 ± 1.4E-04* | 1.309 ± 0.327 | 0.911 ± 0.148 | 1.433 ± 0.124* | ||
| DC | 1 | 0.494 ± 0.193 | 0.243 ± 0.044* | 0.768 ± 0.337 | 0.358 ± 0.062* | 4.535 ± 0.449* | ||
| D | 2.573 ± 1.057 | 1.175 ± 0.486 | 0.076 ± 0.005 | 59.850 ± 6.585* | 1.279 ± 0.219 | 7.027 ± 1.629 | ||
| RC | 0.599 ± 0.231 | 0.385 ± 0.192* | 0.367 ± 0.117* | 0.093 ± 0.033* | 0.276 ± 0.051* | 0.559 ± 0.048* | ||
| R | 2.135 ± 0.757 | 0.662 ± 0.310 | 0.075 ± 0.009* | 0.086 ± 0.009* | 0.003 ± 7.3E-04* | 2.804 ± 0.483 | ||
| SAG:IPT | BS | ND | 0.504 ± 0.177* | 0.756 ± 1.4E-04* | 0.316 ± 0.079* | 0.790 ± 0.129* | 1.427 ± 0.123* | |
| DC | 0.206 ± 0.073 | 0.753 ± 0.323* | 0.310 ± 0.065* | 1.148 ± 0.737 | 0.416 ± 0.097* | 4.721 ± 1.895* | ||
| D | 0.283 ± 0.101 | 1.223 ± 0.493 | 0.167 ± 0.063 | 5.583 ± 0.621 | 0.679 ± 0.147* | 3.676 ± 0.579 | ||
| RC | 0.072 ± 0.026 | 0.788 ± 0.278 | 0.671 ± 1.2E-04* | 0.364 ± 0.091* | 0.373 ± 0.061* | 1.902 ± 0.164* | ||
| R | 0.607 ± 0.215 | 1.520 ± 0.536* | 0.708 ± 1.3E-04* | 0.566 ± 0.142 | 0.657 ± 0.107* | 2.980 ± 0.257 | ||
| Col-0 CK | BS | 1 | 1 | 1 | 1 | 1 | ||
| DC | 0.712 ± 0.315* | 0.141 ± 0.024 | 4.532 ± 0.539* | 0.424 ± 0.079* | 3.701 ± 0.497* | |||
| D | 0.458 ± 0.161 | 0.048 ± 9.0E-06* | 3.671 ± 0.089* | 0.477 ± 0.078* | 1.097 ± 0.272* | |||
| RC | 0.732 ± 0.258 | 0.436 ± 8.1E-05* | 0.253 ± 0.063* | 0.326 ± 0.053* | 0.871 ± 0.075* | |||
| R | 1.203 ± 0.424 | 0.755 ± 1.4E-04* | 0.730 ± 0.183 | 0.468 ± 0.076 | 1.327 ± 0.114* |
HvCKX2, cytokinin oxidase/dehydrogenase 2 from Hordeum vulgare; IPT Agro, isopentenyl transferase from Agrobacterium; CKX1, cytokinin oxidase/dehydrogenase 1; IPT3, isopentenyl transferase 3; RD29B, responsive to desiccation 29B; P5CS1, δ-1-pyrroline-5-carboxylate synthase 1; NCED3, 9-cis-epoxycarotenoid dioxygenase 3. The expression was determined before stress application–BS (25 DAS); in well-watered plants at the end of drought period–DC (38 DAS); in drought stressed plants–D (38 DAS); in well-watered plants at the end of recovery period–RC (44 DAS); and in re-watered plants–R (44 DAS). Transcription values represent means ± SD (n = 6) normalized to WT before stress (25 DAS). Statistically significant (p < 0.05, two-sample t-test) differences between the corresponding variants of WT and transformants are marked (
), and between D/DC or R/RC variants within each genotype (bold). NA, not analyzed; ND, not detected.