Fig. 2. Brightfield images of the artery-on-a-chip after seeding with cells. ECs and VSMCs are seeded separately (A and B, respectively), resulting in both cell types at each side of the membrane (C and D). The ECs can be seen in the upper layer and VSMCs in the lower layer (C). Scalebar represents 50 μm and inserts represent a higher magnification of ECs and VSMCs.