Fig. 7. Live staining of vessel wall on a chip device. A–D. Images were taken directly after adhesion of VSMCs (B) and ECs (C) whereby A and D are merged images. E–H. Live cell stainings after 4 days of dynamic culturing, VSMCs (F), ECs (G) and merged images (E and H). ECs are stained in green and VSMCs in red. Scalebar represents 100 μm, n = 3. I. Cell orientation analysis for ECs in static (blue) and dynamic cultures (red). J. Cell orientation analysis for VSMCs in static (blue) and dynamic (red) cultures. Cell orientation analysis is analysed by the CTG/CTO and F-actin images, n = 4–6.
