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. Author manuscript; available in PMC: 2019 May 21.
Published in final edited form as: Dev Cell. 2018 May 21;45(4):450–464.e3. doi: 10.1016/j.devcel.2018.04.020

Figure 3. Genetic and physical interaction between Myo1D and Dronc.

Figure 3

(A–D) Head capsule phenotypes of the indicated genotypes. Scale bar: 200μm.

(E) Extracts from ey>hid,p35,GFP and ey>hid,p35;Myo1D-GFP eye/antennal imaginal discs were immunoprecipated with anti-GFP antibodies. Shown are immunoblots of Myo1D-GFP immunoprecipitates probed with anti-Dronc (SK11) (top) and anti-GFP antibodies (bottom). Arrows indicate Myo1D-GFP and Dronc.

(F) Extracts from ey-Gal4 (-), ey>Flag-Dronc and ey>Flag-Dronc,Myo1D-HA eye/antennal imaginal discs were immunoprecitated with anti-Flag antibodies. Shown are immunoblots probed with anti-HA antibodies to visualize Myo1D-HA (top, arrow) and with anti-Flag antibodies to reveal Flag-Dronc (bottom).

(G) Autoradiographs of in vitro cleavage assays of radio-labeled DrICE (positive control) and radio-labeled Myo1D by unlabeled active Dronc. Asterisks mark the cleavage products of DrICE.

(H) Immunoblot analysis of total extracts from ey>hid,p35;Myo1D-GFP eye/antennal imaginal discs using anti-GFP antibodies.