KEY RESOURCES TABLE.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal NimC (1:300) | Kindly provided by I. Ando | (Kurucz et al., 2007) |
| Guinea pig Dronc (1:200) | Kindly provided by P. Meier | SK11; (Wilson et al., |
| Rabbit polyclonal Cleaved Caspase 3 (CC3) (1:400) | Cell Signaling Technology | Cat#9661S |
| Mouse monoclonal Wg (1:50) | DSHB | Cat#4D4 |
| Mouse monoclonal Myo1D (1:250) | Abmart, Shanghai, China | Cat#20735-1-16/P23_141121 |
| Rabbit polyclonal Duox (1:100) | LS Bio | Cat#C410118 |
| Rabbit polyclonal Cleaved Dcp1 (cDCP1) (1:200) | Cell Signaling Technology | Cat#9578S |
| Mouse monoclonal β-Gal (1:100) | Promega | Cat#Z378A |
| Rabbit polyclonal Phospho-JNK (pJNK) (1:200) | Promega | Cat#V793B |
| Mouse monoclonal GFP (Western Blot-1:1000) | Pierce | Cat#MA15256 |
| Rat polyclonal ELAV (1:50) | DSHB | Cat#7EBA10 |
| Mouse monoclonal HA (1:200, Western Blot-1:2000) | Invitrogen | Cat#26183 |
| Mouse monoclonal FLAG (Western Blot-1:1000) | Sigma-Aldrich | Cat#F1804 |
| Bacterial and Virus Strains | ||
| E. coli strain BL21pLysS(DE3) | Promega | Cat#L1191 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| DHE | Invitrogen | Cat#D23107 |
| Gfp-Trap_M beads | Chromotek | Cat#Gtm-20 |
| anti-Flag M2 magnetic beads | Sigma-Aldrich | Cat#M8823 |
| 6x-His-Dronc protein | Kindly provided by G. Salvesen | (Snipas et al., 2008) |
| DrICEC211A | Kindly provided by G. Salvesen | (Snipas et al., 2008) |
| Vectashield with DAPI | Vector Laboratories | Cat#H-1200 |
| Critical Commercial Assays | ||
| Rabbit reticulocyte lysates | Promega | Cat#L4610 |
| In Situ Cell Death Detection Kit, TMR red | Roche | Cat#12156792910 |
| Experimental Models: Organisms/Strains | ||
| UAS-hid; ey-Gal4 UAS-p35/CyO,tub-Gal80 | (Fan et al., 2014) | |
| ey-Gal4 UAS-p35/CyO | (Fan et al., 2014) | |
| scrib−/− RasV12 | Kindly provided by M. Kango-Singh | (Chen et al., 2012) |
| Myo1D RNAi | Bloomington Drosophila Stock Center | BDSC33971 FlyBase: FBst0033971 |
| Myo1D RNAi | Vienna Drosophila RNAi Center | v102456 FlyBase:FBst0475947 |
| Myo1DK2 | Kindly provided by S. Noseli | FlyBase:FBal0194493 (Speder et al., 2006) |
| Myo1DEy08859 | Bloomington Drosophila Stock Center | FlyBase:FBst0019940 |
| UAS-Myo1D | Kindly provided by S. Noseli | (Speder et al., 2006) |
| UAS-Myo1D-GFP | Kindly provided by S. Noseli | (Speder et al., 2006) |
| UAS-Myo1D-HA | Kindly provided by K. Matsun, | FlyBase:FBtp0081821 (Hozumi et al., 2008) |
| UAS-Myo1C-HA | Kindly provided by K. Matsuno | (Hozumi et al., 2008) |
| UAS-Myo1D/1C-HA | Kindly provided by K. Matsuno | FlyBase:FBtp0081835 (Hozumi et al., 2008) |
| UAS-Myo1C/1D-HA | Kindly provided by K. Matsuno | (Hozumi et al., 2008) |
| UAS-Myo1D-HAmATP | Kindly provided by K. Matsuno | FlyBase:FBtp0081822 (Hozumi et al., 2008) |
| UAS-Myo1D-HAΔAbs | Kindly provided by K. Matsuno | FlyBase:FBtp0081824 (Hozumi et al., 2008) |
| UAS-Myo1D-HAΔIQ | Kindly provided by K. Matsuno | FlyBase:FBtp0081825 (Hozumi et al., 2008) |
| UAS-Myo1D-HAΔtail | Kindly provided by K. Matsuno | FlyBase:FBtp0081826 (Hozumi et al., 2008) |
| UAS-Flag-dronc | Bergmann lab | FlyBase:FBtp0093662 (Kamber Kaya et al., 2017) |
| UAS-dark-GFP | Kindly provided by H-D Ryoo | FlyBase:FBtp0116994 (Shapiro et al., 2008) |
| UAS-dronc RNAi | Kindly provided by P. Meier | BDSC32963 FlyBase:FBst0032963 (Leulier et al., 2006) |
| UAS-Duox RNAi | Kindly provided by W. J. Lee | FlyBase:FBal0190061 (Ha et al., 2005) |
| UAS-Duox | Kindly provided by W. J. Lee | FlyBase:FBal0190278 (Ha et al., 2005) |
| droncI24 | Bergmann lab | FlyBase:FBal0190335 (Xu et al., 2005) |
| dcp1Prev | Kindly provided by K. McCall | FlyBase:FBal0156746 (Laundrie et al., 2003) |
| drICEΔ1 | Kindly provided by B. Hay | FlyBase:FBal0193685 (Muro et al., 2006) |
| DE-Gal4 | Kindly provided by G. Halder | (Morrison and Halder, 2010) |
| UAS-hid/+; DE-Gal4 tub-Gal80ts/+ | Bergmann lab | (Fan et al., 2014) |
| Nub-Gal4 | (Calleja et al., 1996) | |
| GMR-Gal4 UAS-eiger | Kindly provided by M. Miura | (Igaki et al., 2002) |
| UAS-bsk RNAi | Vienna Drosophila RNAi Center | v34138 FlyBase:FBst0460476 |
| UAS-Traf2 RNAi | Vienna Drosophila RNAi Center | v110266 FlyBase:FBst0481844 |
| puc-lacZ | FlyBase:FBal0047865 (Ring and Martinez Arias, 1993) | |
| GMR-hid | Kindly provided by H. Steller | FlyBase:FBal0265023 (Grether et al., 1995) |
| UAS-Empty Vector (UAS-EV) | Bergmann lab | (Kamber Kaya et al., 2017) |
| Canton S | FlyBase:FBst0064349 | |
| w1118 | FlyBase:FBst0003605 | |
| w*; KrIf-1/CyO; M{lexAop-UAS-GrabFP.A.Int.mCh}ZH-86Fb/TM6B, Tb1 | Bloomington Drosophila Stock Center | FlyBase:FBst0068178 (Harmansa et al., 2017) |