FIG. 5.

SMC-selective p53 transgenic overexpression inhibits injury-induced neointimal formation in vivo. (A) A schematic diagram of the SMMHC-p53 transgenic expression cassette. The p53 cDNA was cloned into the expression vector, which carries SMMHC promoter and the BGH polyadenylate DNA fragments. (B) P53 was overexpressed in SMC tissues of transgenic mice. (C) Representative photomicrographs from elastica-van Gieson-stained sections. Images showed the neointimal development of mouse arteries with 14 days after wire-mediated injury in wild-type or SMMHC-p53 mice (left). Relative intima/medial ratios were calculated by measuring area of each vascular layer and lumen (n = 6, ***P < 0.05) (right). Color images available online at www.liebertpub.com/scd