Table 1.
Parameters considered for characterization of the reference materials produced during the pilot service implemented in PRO-METROFOOD.
| Characterised Parameter | Applied Methods | |
|---|---|---|
| Nutrients and bioactive compounds | Sugars, carbohydrates | HPLC-RI, Ewers method |
| Fibers (total, crude, dietetic; amylose content) | Enzymatic method, Henneberg-Stohmann method, Flow Injection Spectrophotometric Analysis | |
| Mineral salts (Ca, K, Na, Mg, P) and trace elements | AAS, ICP-AES, ICP-MS | |
| Vitamins (C, B group) | LC-MS/MS | |
| Lipids | Extractive gravimetry | |
| Fatty acids | GC-FID | |
| Protein fractions | Kjeldahl method, LC-MS/MS, Spectroscopy (Bradford) | |
| Amino acids | UPLC-PDA | |
| Phenols | Spectrophotometric analysis, HPLC, LC-MS/MS | |
| Tocols | HPLC-DAD | |
| Flavonoids and flavonols | Spectrophotometric analysis | |
| Bioactivity and reactivity | Antioxidant activity | Fluorimetric analysis, UV-Vis Analysis, DPPH scavenging assay |
| Organic Contaminants and Residues | Toxigenic fungi and toxins | LC-MS/MS, HPLC-FLD, ELISA |
| Pesticides, pharmaceutical residues and veterinary drugs | GC-MS, GC-ECD, LC-MS | |
| Inorganic Contaminants | Toxic elements (e.g., As, Cd, Cu, Hg, Ni, Pb) | ET-AAS, AMA, ICP-AES, ICP-MS, ID-ICP-MS, MC-ICP-MS, TXRF, INAA |
| Speciation (As speciation, Hg and MeHg, Sn, and organotin compounds) | HPLC-ICP-MS, GC-ICP-MS | |
| Isotopes (e.g., Hg and Sr isotopes, stable isotope analysis of light elements) | MC-ICP-MS, EA-IRMS | |
| Microbiological analysis (e.g., membrane filtration; occurrence of Campylobacter jejuni, C. coli, enterohemorrhagic Escherichia coli, Salmonella spp., Vibrio parahaemolyticus, V. vulnificus, emetic type of food-borne Bacillus Cereus species group) | Membrane filtration, accumulation techniques, fluorescence | |
| Physical characterization (e.g., ash, particle size, gelatinization-retrogradation, texture parameter of cooked rice) | Gravimetry for ash; transmission electronmicroscopy, scanning electronmicroscopy for particle size; differential scanning calorimetry for gelatinization-retrogradation | |
| Allergen profile characterization | Gel electrophoresis, LC-MS/MS characterization and protein identification by advanced bioinformatic tools | |
| Genetic Analyses | Genotyping | Feedcode TBP (Tubulin Based Polymorphism) |
| Rice endogenous/GM construct screening* | PCR | |
*
only for rice grains and rice flour.