Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Apr 30;12(1):137–152. doi: 10.1080/19336950.2018.1457898

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 1. — Expression, purification, and initial crystallization of rat TRPV6. (a) Map of the BacMam vector for rTRPV6 expression. The rTRPV6 construct has a C-terminal thrombin cleavage site followed by eGFP and a Strep affinity tag. The vector includes polyhedrin and CMV promoters, an ampicillin resistance selection marker, Tn7R transposition sites and an SV40 polyadenylation signal. (b) FSEC profiles for rTRPV6-C2 and rTRPV6-C2-L495Q constructs expressed in HEK 293 cells. (c–d) An image of an rTRPV6-C2-L495Q crystal in a hanging drop (c) and its diffraction pattern (d).