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. 2018 Apr 30;12(1):137–152. doi: 10.1080/19336950.2018.1457898

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© 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Crystallization of the RAD-NQ construct. (a) Example of an optimized RAD-NQ crystal flash frozen in a cryo-loop. The red box indicates boundaries of the X-ray beam. (b) 3.85 Å-resolution diffraction pattern for the RAD-NQ crystal. Inset is a magnified view of the diffraction pattern (red boxes). (c) Side view of the RAD-NQ tetramer that represents the content of the asymmetric unit. Each subunit is colored differently. Blue mesh represents the electron density map at 1.0 σ. (d–e) Close-up views of the electron density for the (d) ankyrin repeat and (e) transmembrane domains.