FIG 5.

Ifng and Aid mRNA expression after infection. WT, AID^−/−, sIgM^−/−, and AID^−/− sIgM^−/− (DKO) mice were inoculated intracerebrally with 1,000 PFU SINV TE. RNA was extracted from brain and spinal cord homogenates, and RT-qPCR was performed to determine the change (fold increase) in Ifng mRNA expression in brains (A) and spinal cords (B) from that before infection (day 0). Because AID^−/− and DKO mice do not express the AID gene, only WT and sIgM^−/− mice were tested for Aid mRNA expression in brains (C) and spinal cords (D). *, P < 0.05; ***, P < 0.001.