FIG 5.

Sequential IFN-γ T cell reactivity to ZIKV or DENV LFn fusion proteins in ZIKV or DENV subjects. Preinfection, recent-, and convalescent-phase PBMC samples from ZIKV (A) or DENV (B) IgM-positive subjects were subjected to homologous LFn NS3 protease (ZIKV, red-shaded circle; DENV, blue-shaded circle) and helicase (ZIKV, red-outlined circle; DENV, blue-outlined circle) protein stimulation in IFN-γ LFn ELISPOT ex vivo experiments. Responses for each time point are expressed as the number of secreting cells per 10⁶ PBMCs. Months PI, months postinfection. *, preinfection versus recent, P < 0.05; **, recent versus convalescent time point 1, P < 0.05; ***, convalescent time point 2 versus time point 3, P < 0.05.