FIG 7.

T cell-specific and cross-reactive NS3 responses in the recent and convalescent phases of ZIKV or DENV infections. Recent- and convalescent-phase PBMC samples from ZIKV or DENV subjects were treated with homologous and heterologous LFn-ZIKV and -DENV NS3 protease and helicase proteins, and the specific IFN-γ⁺ and TNF-α⁺ T cells were detected by LFn ELISPOT ex vivo experiments. (A to C) Representative image and magnitude of recent ZIKV/DENV infection-phase responses (A and B) and magnitude of convalescent-phase responses (C) from ZIKV subjects. (D to F) Representative image and magnitude of recent DENV/ZIKV infection-phase responses (D and E) and magnitude of convalescent responses from DENV subjects (F). Shown is the average magnitude of response of individual subjects to either LFn-ZIKV or -DENV NS3 protease (purple-shaded circle) and helicase (purple-outlined circle) proteins. Dotted lines represent the cutoff value. *, P < 0.05.