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. 2018 Mar 14;92(7):e01997-17. doi: 10.1128/JVI.01997-17

TABLE 1.

Primers used for RT-PCR and qRT-PCR

Name Target Orientation Sequence (5′–3′) Purpose
HSLI-F HCV domain I Forward GACGCGACCTGCCCCTAATAG RT-PCR
HSLIII-R HCV domain III Reverse ACCCAGTCTTCCCGGCAA RT-PCR
ESLI-F EHcV domain I Forward TACGACACCTCCGTGCTATGCA RT-PCR
ESLI′-F EHcV domain I′ Forward GGAGGGATTCTTCCACATTA RT-PCR
ESLIII-R EHcV domain III Reverse TCGGCTCCGAAGGTCACG RT-PCR
NS3-F HCV NS3 Forward CTGCTTATGCCCAGCAAACA RT-PCR or qRT-PCR
NS3-R HCV NS3 Reverse AACGTCGAGTGTCTCAACGG RT-PCR or qRT-PCR
GAP-F GAPDH Forward GAAGGTGAAGGTCGGAGTC RT-PCR or qRT-PCR
GAP-R GAPDH Reverse GAAGATGGTGATGGGATTTC RT-PCR or qRT-PCR