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. 2018 Mar 14;92(7):e02152-17. doi: 10.1128/JVI.02152-17

FIG 5.

FIG 5

Deletion of VACV WR 199 (B18R), the homolog of the 68k-ank gene, does not impair postreplicative gene expression. (A) Diagram showing replacement of VACV ORF B18R with the GFP gene regulated by the VACV P11 late promoter. (B) The indicated cells were infected with 0.5 PFU/cell of parental VACV WR strain (WR) or VACV WR ΔB18R (ΔB18R) for 16 h. The proteins were resolved by SDS-PAGE, transferred to membranes, and probed with antibodies to A3 intermediate/late protein (upper row) and early I3 protein (middle row). Actin (lower row) served as a loading control. (C) The indicated cells were infected with 0.5 PFU/cell of MVA, MVA-GFP, MVA-Δ186, and recombinant rMVAs 44/47.1, 44.2, 51.1, 44/47.1-Δ186, 44.2-Δ186, and 51.1-Δ186 for 16 h. Proteins were resolved by SDS-PAGE, transferred to membranes, and probed with antibodies to the A3 protein and actin.