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. 2018 Mar 14;92(7):e02152-17. doi: 10.1128/JVI.02152-17

FIG 7.

FIG 7

C5L and M2L ORFs rescue the postreplicative gene expression defect of MVA-Δ186. (A) Diagram representing the MVA-Δ186 genome with position of cosmid 51 and site of GFP replacement of MVA ORF 186. The enlargement shows missing ORFs within deletion I (C17-C12), deletion V (C5-C1), and deletion II (M1-K1). (B) Diagram representing the genome of MVA-Δ186 and DNA containing the mCherry ORF regulated by the P11 promoter, followed by an insert containing C17-C12, C5-C1, or M1-K1 DNA flanked by sequences to enable homologous recombination. The Western blot shows A3 protein and an actin loading control from cells infected for 16 h with 0.5 PFU/cell of MVA-Δ186, rMVA-Δ186-C17-C12, rMVA-Δ186-M1-K1, or rMVA-Δ186-C5-C1. (C) Diagram representing the genome of MVA-Δ186 and DNA containing the mCherry ORF regulated by the P11 promoter, followed by an insert containing an individual ORF (C1, C2, C3, C5, K1, M1, or M2) regulated by the mH5 promoter and flanked by sequences to enable homologous recombination. The Western blot shows A3 protein and an actin loading control from cells infected for 16 h with 0.5 PFU/cell of MVA, MVA-Δ186, or rMVA-Δ186 constructs expressing C1, C2, C3, C5, K1, M1, or M2 protein.