FIG 6.

Disruption of hnRNP-vRNA interactions does not affect the accumulation of SINV vRNAs. (A) 293HEK cells were infected with either wild-type parental virus or the individual hnRNP interaction site mutants at an MOI of 10 PFU/cell. At 12 h postinfection, the total cellular RNA was extracted and assessed for the absolute quantities of the genomic, subgenomic, and minus-strand vRNAs by qRT-PCR. (B) Comparative analyses of the individual viral RNA species relative to wild-type virus. (C and D) Identical to panels A and B, except that the A. albopictus C6/36 cell line was utilized. All the data shown are means of three independent biological samples, with the error bars representing standard deviations of the mean.