FIG 4.

The σ1s protein is dispensable for reovirus infectivity. (A) SVECs were infected with rsT1L or rsT1L σ1s-null at an MOI of 10 or 100 PFU/cell. At 6 h, cells were treated with 10 mM ammonium chloride to prevent secondary rounds of infection. At 24 h, viral protein was visualized by immunofluorescence staining for reovirus antigen. Nuclei were stained with DAPI. (B) The percentages of infected cells in panel A were quantified, and the results are presented as the mean percentage of infected cells from three independent experiments for each condition. Error bars indicate standard deviations. (C) SVECs were infected with rsT1L or rsT1L σ1s-null ISVPs at the indicated MOIs and were processed as described for panel A. The percentages of infected cells were determined, and the results are presented as the mean percentage of infected cells from three independent experiments for each condition. (D) SVECs were infected with rsT1L or rsT1L σ1s-null at the indicated MOIs. After 1 h of adsorption, the SVECs were washed, trypsinized, and counted, and 1,000 cells per well were first plated on monolayers of L929 fibroblasts and then processed for plaque assays. Results are presented as the mean number of infectious centers per 1,000 cells from three independent experiments for each condition.