FIG 5.

The σ1s protein is dispensable for reovirus mRNA synthesis. SVECs were infected with rsT1L or rsT1L σ1s-null at an MOI of 10 PFU/cell. Total RNA was purified at the indicated times postinfection. (A and B) The relative quantity (RQ) of reovirus S4 positive-sense (A) or negative-sense (B) RNA compared to the quantity at the 0-h time point was determined by RT-qPCR using a TaqMan assay. (C) mRNA levels for the L1, L2, L3, M1, M2, M3, S1, S2, and S3 genes. Positive- and negative-sense RNA levels for these gene segments were determined by RT-qPCR using SYBR green. The RQ of positive- or negative-sense RNA was determined with reference to the quantity at the 0-h time point. The RQ of reovirus mRNA was determined by subtracting the RQ of negative-sense viral RNA (representing genomic RNA production) from the RQ of positive-sense RNA. Data are presented as the mean log₂ RQ from three independent experiments. Error bars indicate standard deviations. *, P < 0.05 (as determined by Student's t test).