FIG 7.

The σ1s protein does not affect reovirus protein stability. SVECs were either mock infected or infected with rsT1L or rsT1L σ1s-null ISVPs at an MOI of 1 PFU/cell. At 13 h, either dimethyl sulfoxide (used as a vehicle control [VC]) or cycloheximide (CHX; 25 μg/ml; used to halt protein synthesis) was added to the culture medium. Cell lysates were collected at the indicated times posttreatment, and proteins were separated by SDS-PAGE. (A, B, D, and E) Reovirus outer capsid protein μ1 (A and B) or nonstructural protein σNS (D and E) was detected by immunoblotting. Upper bands in panels A and B represent full-length μ1 protein. (C and F) Densitometry was performed on the full-length μ1 protein (C) and the σNS protein (F) to determine the rate of protein degradation by linear regression analysis. Results are presented as the average relative quantity of protein from two independent experiments. Error bars indicate standard deviations.