FIG 8.

The σ1s protein influences VF morphology in SVECs. (A) SVECs were infected with rsT1L or rsT1L σ1s-null at an MOI of 100 PFU/cell. At the indicated times, cells were fixed and were stained for reovirus nonstructural protein σNS (green) and nuclear DNA (DAPI) (blue). (B) Colored arrows indicate the four staining patterns identified: large/filamentous (blue), small/globular (red), punctate (green), and diffuse (purple). (C) At least 100 infected cells were counted and were classified by the staining patterns of the VFs. Data are presented as the average percentage of cells exhibiting each indicated staining pattern from three independent experiments with standard deviations.