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. 2018 Mar 14;92(7):e02009-17. doi: 10.1128/JVI.02009-17

FIG 3.

FIG 3

miR-130a gRNA and a miR-130a inhibitor increased PKLR expression and HCV replication. A miR-130a hairpin inhibitor or CRISPR/Cas9 miR-130a gRNA was used to knock down miR-130a expression. The miR-130a inhibitor, negative-control inhibitor, miR-130a gRNA, and negative-control gRNA were transfected into Huh7.5.1 cells. HCV JFH1 was inoculated into the appropriate wells and incubated for 48 h. (A) miR-130a gRNA decreased the miR-130a level. (B) miR-130a gRNA increased the PKLR mRNA level. (C) miR-130a gRNA promoted HCV RNA replication. (D) miR-130a gRNA increased PKLR and HCV core protein levels. (E) miR-130a gRNA or the miR-130a inhibitor did not affect cell viability. (F) The miR-130a inhibitor decreased the miR-130a level. (G) The miR-130a inhibitor increased the PKLR mRNA level. (H) The miR-130a inhibitor promoted HCV RNA replication.