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. 2018 Mar 14;92(7):e02009-17. doi: 10.1128/JVI.02009-17

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Copyright © 2018 Duan et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 7 — PKLR regulated HBV replication. (A) PKLR overexpression increased the HBV DNA level in HepAD38 cell supernatant. (B) HBV replication in HBV-infected NTCP-Huh7.5.1 cells increased in a time-dependent manner. PKLR gRNA lowered HBV DNA levels significantly compared to those with the negative-control gRNA, in a time-dependent manner. (C) PKLR gRNA reduced the HBV DNA level in cell culture supernatant. (D) PKLR gRNA reduced the HBV cccDNA level in HBV-infected NTCP-Huh7.5.1 cells. (E) PKLR gRNA reduced the HBV DNA level in HBV-infected NTCP-Huh7.5.1 cells.