FIG 6.

Knockdown of CK1α results in impaired degradation of IFNGR1 and IFNAR1 during IAV infection or HA expression. (A and B) HEK293 cells were transfected with siRNA specific to CK1α (siCK1α) or nonspecific scrambled control siRNA (SCR). At 24 h posttransfection, cells were left uninfected (Mock), infected with IAV (A), or infected with IAV-pH1N1 (B) at an MOI of 1. Twenty-four hours later, the levels of IFNGR1, IFNAR1, CK1α, viral NP, viral M1, and GAPDH were analyzed by Western blotting. (C) HEK293 cells were transfected with siCK1α or SCR. At 24 h posttransfection, cells were transfected with a control vector or plasmids encoding HA. Western blotting was performed 24 h after HA transfection, and the levels of IFNGR1, IFNAR1, CK1α, HA, and GAPDH were detected. Mass, molecular mass (kilodaltons).