FIG 1.

HAdV-41 infection of EC cells. (A) EC cells infected with HAdV-41 at 18 hpi. Infected (left) and uninfected (right) cells were visualized by confocal microscopy. (B) Western blot analysis of purified HAdV-41-infected cell lysate at 6 and 24 hpi and uninfected cells (UN). In the purified HAdV-41 lysate, the hexon protein is 100 kDa, the long fiber and penton base proteins are in the range 60 to 65 kDa, and the short fiber protein is in the range 40 to 45 kDa. (C) To investigate if HAdV-41 can replicate in human EC cells, a time kinetics study using qPCR was conducted, and as shown EC cells are nonpermissive for replication of HAdV-41. Ct, cycle threshold.