FIG 4.

HAdV-41 short fiber knob and hexon proteins stimulate serotonin release. (A) EC cells were stimulated with purified HAdV-41 penton base protein. The serotonin content in supernatant was analyzed after 6 h of incubation. (B) EC cells were stimulated with 2 μM purified HAdV-41 fiber knob proteins, Serotonin content in supernatant was analyzed by ELISA after 6 h of incubation. (C) EC cells were stimulated with purified HAdV-41 hexon protein. Serotonin content in supernatant was analyzed by ELISA after 6 h of incubation. (D) SDS-PAGE of purified short and long fiber knob, penton base, and hexon. The diluent control was PBS. DLP, purified rotavirus double-layer particle. ***, P < 0.001, and *, P < 0.05, by Student's t test. Data are means ± SEM (n = 4).