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Determination of HIV-1 Env expression by capture ELISA. HEK293T cells were transfected with plasmids expressing wild-type (WT) or mutant HIV-1NL4-3 Envs. The glycoproteins (gp120/gp41) in the lysates of transfected cells (A) or culture supernatant (B) were captured by sheep anti-gp120 antibody D7324 and detected with human MAbs VRC01 and 10E8. The experiments were conducted in duplicate and repeated 2 times.
