FIG 8.

poMx1 proteins undermine the RdRp activity of NS5B proteins. RdRp assays were performed as follows: with increasing amounts of NS5B proteins (0, 50, 100, 150, 200, 250, and 300 ng) (A); with 100 ng of NS5B proteins and increasing amounts of poMx1 proteins (WT) (0, 50, 100, 150, 200, 250, or 300 ng) (B); with 100 ng of NS5B proteins and 100 ng of poMx1 proteins (WT and mutant) with the RNA templates (C). Quantification of synthesized RNA was determined using RT-qPCR as described in Materials and Methods. The results showed that newly synthesized RNA products were detected. Results are presented as the means ± SD of data from three independent experiments. **, P < 0.01.