TABLE 3.

Relative replication capacity of SIV_mac239 single-point substitutions and in-frame deletions^a

Mutation	Region	Rhesus 221 cells			Rhesus 444 cells
		RRC	R2	P value	RRC	R2	P value
S132P	V1 loop	−0.131	0.373	0.061	−0.040	0.063	0.432
T136N	V1 loop	−0.206	0.020	0.664	−0.083	0.269	0.084
A138T	V1 loop	−0.173	0.073	0.396	−0.036	0.051	0.482
S139P	V1 loop	−0.087	0.027	0.609	0.056	0.086	0.380
V142I	V1 loop	−0.224	0.015	0.705	−0.011	0.005	0.830
A417T	V4 loop	0.040	0.028	0.604	−0.023	0.015	0.704
P421Q	V4 loop	0.152	0.670	0.001	0.078	0.064	0.427
P421S	V4 loop	−0.172	0.352	0.042	−0.173	0.349	0.043
P421T	V4 loop	0.008	0.004	0.842	0.045	0.014	0.711
ΔV4 1	V4 loop	−0.046	0.014	0.719	0.050	0.016	0.692
ΔV4 2	V4 loop	−0.292	0.242	0.104	−0.463	0.434	0.020

^aThe RRC of single-point and multiple-amino-acid deletion mutants was calculated by linear regression analysis of mean frequency values. The R² value of each line and the P value of an F-test, testing whether the slope of the line is significantly different than zero, is reported. Significant P values (<0.05) are reported in boldface.