FIG 7.

Association of K8 with ori-Lyt DNA is mediated by RNA. (A) Schematic illustration of the KSHV ori-Lyt core domain and DNA fragments that were used in the DNA affinity assay. (B) Three biotinylated ori-Lyt DNA fragments and an irrelevant DNA fragment from the ORF45 coding region as a control were prepared by PCR, conjugated on magnetic beads, and incubated with TPA-induced BCBL-1 nuclear extract with and without treatment with RNase A. After washing, samples were assayed by Western blotting with antibodies as indicated. (C) Binding of K8 to ori-Lyt DNA was determined in BAC16 (BAC WT) and BAC-K8GDDGR by ChIP assay with anti-K8 antibody. The positions of the amplicons (3.1F and 12F) are shown in panel A. The error bars indicate SD.