FIG 2.

Spatiotemporal organization of DENV replication and translation. (A) Abundance of DENV plus- and minus-strand RNA over a 40-h infection time course, as assessed by RNA-seq. (B) Rate of accumulation for DENV plus- and minus-strand RNA. Each point indicates the average rate of change of RNA abundance between the two adjacent time points, expressed as percent change per hour in an exponential growth model. (C) Percentage of DENV plus-strand RNA, minus-strand RNA, and plus-strand translation that is ER associated throughout the experimental time course. (D) Translational efficiency of DENV RNA relative to that of the host transcriptome. The translation efficiency distributions of host mRNAs encoding TMHMM-predicted ER-targeted proteins are shown in black, with the translation efficiency of DENV RNA in red. The translation efficiency distribution is calculated as an average value of all mRNAs at all time points. For all panels, error bars represent ± standard deviations (n = 2).