FIG 5.

Comparison of DKK1 expression during the BoHV-1 latency-reactivation cycle. TG were collected from 3 latently infected calves (A) or latently infected calves treated with DEX for 6 h to initiate reactivation from latency (B). IHC was performed as described in Materials and Methods using a DKK1-specific antibody (ab188597; Abcam); arrows denote DKK1⁺ TG neurons, and closed circles denote extracellular DKK1 staining. Magnification is approximately ×400, and these sections are representative of many sections that were examined.