TABLE 1.
Sampleb | No. of raw reads | No. of reads after trimming | No. of mapped reads | Mapping (%) |
|
---|---|---|---|---|---|
Concordant pair | Overall | ||||
Latency (1) | 87,772,072 | 86,899,588 | 80,341,469 | 84.90 | 91.53 |
Latency (2) | 113,935,336 | 113,602,597 | 103,298,949 | 83.70 | 90.66 |
Latency (3) | 103,295,710 | 102,763,023 | 94,102,756 | 84.60 | 91.10 |
30 min (1) | 130,998,440 | 130,868,656 | 68,976,317 | 49.80 | 52.65 |
30 min (2) | 134,891,362 | 134,664,406 | 46,302,068 | 32.20 | 34.33 |
1.5 h (1) | 181,545,774 | 181,349,993 | 97,355,270 | 50.60 | 53.63 |
1.5 h (2) | 118,128,452 | 117,968,772 | 109,163,219 | 86.50 | 92.41 |
1.5 h (3) | 78,031,304 | 77,912,973 | 71,808,615 | 86.30 | 92.03 |
3 h (1) | 20,268,356 | 20,254,807 | 19,210,229 | 90.10 | 94.78 |
3 h (2) | 61,588,174 | 61,550,670 | 58,667,117 | 91.00 | 95.26 |
3 h (3) | 193,385,058 | 193,130,128 | 181,472,604 | 88.90 | 93.84 |
Uninfected | 146,102,758 | 145,870,066 | 134,616,584 | 86.20 | 92.14 |
Summary of reads for RNA-sequencing results during latency (3 calves). Reads for latently infected calves treated with DEX for 30 min (2 calves), 1.5 h after DEX treatment (3 calves), and 3 h after DEX treatment (3 calves) are also provided. As a control, RNA sequencing was performed from an uninfected calf.
The numbers in parentheses denote TG from different animals.