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. 2017 Apr 28;9(1):72–79. doi: 10.1080/21655979.2017.1304872

Figure 3.

Figure 3.

Effect of temperature and pH on enzyme activity and stability. (A) Effect of temperature on enzyme activity. The maximum activity at 35°C was taken as 100%. (B) Effect of temperature on enzyme stability. Enzymes were incubated for 1 h at the indicated temperatures. The samples were then measured under standard conditions. The activity without treatment was taken as 100%. (C) Time-course thermal stability. The enzyme activities were determined by evaluating residual activities after incubation for 0 min, 10 min, 20 min, 30 min, 40 min, 60 min, 90 min and 120 min at 60°C. The activity without treatment was taken as 100%. (D) Effect of pH on enzyme activity. The enzyme activity was measured at 35°C in different buffers with pH values ranging from 3 to 11. The maximum activity observed in pH 7.0 buffer was taken as 100%. The error bars represent the SD of the mean calculated for 3 replicates. The circles represent IDO, and the squares represent IDOM3.