. 2017 Apr 28;9(1):72–79. doi: 10.1080/21655979.2017.1304872

Table 3.

Primers.

Primers^*	Sequence (5′–3′)	Description
sucA-1	AAGCATAAAAAAGATGCTTAAGGGATCACGTAACGGCTGACATGGGAATTAG^*	Deletion of sucA
sucA-2	TATCTACGCTACTCATTGTGTATCCTTTATGCAACAACAACCGTTGCTGACTGTAGGCCG	Deletion of sucA
sucA-3	ACGTAGACAAGAGCTCGCAAGTG	Identification for sucA deletion
sucA-4	CGGGTTTTTTATGCCAGGTTG	Identification for sucA deletion
aceA-1	AACCACCACATAACTATGGAGCATCTGCACTAACGGCTGACATGGGAATTAG	Deletion of aceA
aceA-2	CGGCCTACAGTCAGCAACGGTTGTTGTTGCGCAACAACAACCGTTGCTGACTGTAGGCCG	Deletion of aceA
aceA-3	TGGAACAGATCACCACTTCCG	Identification for aceA deletion
aceA-4	ATGATAAGACGCGCAAGCGT	Identification for aceA deletion
ido-1	AAGGAAGCTAGATATGAAAATGAGTGGCTTTAGCATAG	Amplification of ido and expressed by pWSK29
ido-2	TTATTTTGTCTCCTTATAAGAAAATGTTACTA	Amplification of ido and expressed by pWSK29
ido-3	ATGAAAATGAGTGGCTTTAGCATAG	Amplification of ido and expressed by pET-His
ido-4	TTATTTTGTCTCCTTATAAGAAAATGTTACTA	Amplification of ido and expressed by pET-His

Underlined letters indicate the sequence 100 bpupstream or downstream of the gene to be deleted.