2.

过量表达circHIPK3促进NCI-H1299细胞增殖。A：circHIPK3在6株NSCLC细胞系中的表达量；B：稳定转染对照空质粒pLDCH和circHIPK后，NCI-H1299细胞表达载体携带的绿色荧光蛋白标记；C：过表达circHIPK后，细胞中circHIPK3及mHIPK3表达水平检测；D：CCK-8试验检测细胞增殖；E：平板克隆形成实验检测细胞增殖；F：克隆形成数目统计；^**：与对照组相比，P＜0.01。

Overexpression of circHIPK3 promoted NCI-H1299 cells proliferation. A: The expression level of circHIPK3 in 6 kinds of NSCLC cell lines; B: The green fluorescent protein (GFP) indicated the successful and stable establishment of pLDCH/p-circHIPK3 NCI-H1299 cell line; C: Detection of circHIPK3 and mHIPK3 expression levels in NCI-H1299; D: The cell growth rate was measured by CCK-8 assay; E: Cell proliferation was assessed by colony formation assay; F: Statistics analysis of colony formation assay; ^**: compared with the control, P < 0.01.