4.

CircHIPK3作为“海绵体”吸附miR-379。A：荧光素酶报告基因实验检测miR-379分别和载体对照组EV、circHIPK3过表达组（circ）、circHIPK3敲低组（si-circ）、突变质粒Luc-circHIPK3组（mut-circ）、miR-inhibitor组（miR-in）及Inhibitor control组（IC）共转入293T细胞中的荧光素酶的相对荧光强度；B：荧光素酶报告基因实验检测miR-379分别和EV、circ、si-circ、mut-circ、miR-in及IC共转入NCI-H1299细胞中的荧光素酶的相对荧光强度；C：转入miR-379 mimics和IGF1 3’UTR后，293T细胞中荧光素酶的相对荧光强度检测；D：转入miR-379 mimics和IGF1 3’UTR后，NCI-H1299细胞中的荧光素酶相对荧光强度。^*：与对照组相比，P＜0.05。^**：与对照组相比，P＜0.01。

CircHIPK3 could sequester miR-379. A: Luciferase relative activity of miR-379 in EV, circ, si-circ, mut-circ, miR-in and IC treated 293T cells; B: Luciferase relative activity of miR-379 in EV, circ, si-circ, mut-circ, miR-in and IC treated NCI-H1299 cells; C: Luciferase relative activity of miR-379 with IGF1 3'UTR in 293T cells; D: Luciferase relative activity of miR-379 with IGF1 3'UTR in NCI-H1299 cells. ^*: compared with the control, P < 0.05. ^**: compared with the control, P < 0.01.