3.

MiR-218靶基因的预测及验证。A：miR-218与Robo1的3’UTR结合示意图；B：干扰miR-218后，Robo1的mRNA变化情况；C：干扰miR-218后，Robo1蛋白的表达变化；D：qPCR检测在HCC4006细胞中转染miR-218 mimics后，Robo1的mRNA表达；E：在HCC4006细胞中转染miR-218 mimics后，Robo1的蛋白表达；F、G：双荧光素酶检测miR-218对Robo1转录活性的影响，其中F：干扰miR-218；G：过表达miR-218。

Predicting and verifying the target genes of miR-218. A: Schematic diagram for binding of miR-218 in 3'UTR of Robo1; B: qPCR analysis of Robo1 expression after Anti-miR-218 transfection; C: The protein level of Robo1 after Anti-miR-218 transfection; D: qPCR analysis of Robo1 expression in HCC4006 cell after transfected with miR-218 mimics; E: The protein level of Robo1 in HCC4006 cell after transfected with miR-218 mimics; F, G: Luciferase reporter assay analysis of the transcription activity of Robo1 in cells transfected with Anti-miR-218 (F) or over-expressed with miR-218 (G).