2.

基因组小鼠肿瘤酶消化悬液细胞分析。EGFR^flx/flx相对于EGFR^wt/wt组，PD-1阳性CD4⁺ T细胞比例降低（A），FoxP3阳性CD4⁺ T细胞比例也明显下降（B）。EGFR^wt/wt组样本PD-1阳性CD4⁺T为77.34%（C），同源EGFR^flx/flx样本PD-1阳性CD4⁺ T为55.33%（D）。^*P＜0.05。

FACS analysis the tumor cell suspensions in the genetic model. EGFR^flx/flx keratinocytes formed tumors vs the EGFR^wt/wt, the proportion of PD-1 positive CD4⁺ T cells decreased (A), FoxP3 positive CD4⁺ T cell ratio was also significantly decreased (B). A sample from EGFR^wt/wt group, the PD-1 positive CD4⁺ T ratio was 77.34% (C), the ratio of EGFR^flx/flx sample PD-1 positive CD4⁺ T was 55.33% (D). ^*P < 0.05.