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Real-time PCR引物及退火温度

Real-time primers and anealing temperatures

Gene	Primers sequences	Anealing temperature (℃)
circUBAP2	F: 5’-AGCCTCAGAAGCCAACTCCTTTG-3’	60
	R: 5’-TCAGGTTGAGATTTGAAGTCAAGAT-3’
CDK6	F: 5’-TCTTCATTCACACCGAGTAGTGC-3’	60
	R: 5’-TGAGGTTAGAGCCATCTGGAAA-3’
Cyclin D1	F: 5’-GGCGGATTGGAAATGAACTT-3’ R: 5’-TCCTCTCCAAAATGCCAGAG-3’	58
P27	F:5’-CATTCCATGAAGTCAGCGAT-3’	58
	R: 5’-CGTCAAACGTAAACAGCTCG-3’
c-IAP1	F: 5’-CATTCCATGAAGTCAGCGAT-3’	59
	R: 5’-TGCTTTTGTTGTGATGGTGG-3’
Bcl-2	F: 5’-CAGCCAGGAGAAATCAAACAG-3’	60
	R: 5’-GACTGAGTACCTGAACCGGC-3’
Survivin	F: 5’-TCCGCAGTTTCCTCAAATTC-3’	60
	R: 5’-GTTGCGCTTTCCTTTCTGTC-3’
Bax	F: 5’-CGGCGAATTGGAGATGAACTG-3’	60
	R: 5’-AGCAAAGTAGAAGAGGGCAACC-3’
FAK	F: 5’-GCTCCACCAAAGAAACCG-3’	56
	R: 5’-GCCCGTCACATTCTCGTAC-3’
Rac1	F: 5’-TGTAGTCGCTTTGCCTATTGATG-3’	59
	R: 5’-CATCGTCAGCACTAGCACAGTTT-3’
MMP2	F: 5’-TGATCTTGACCAGAATACCATCGA -3’	60
	R: 5’-GGCTTGCGAGGGAAGAAGTT-3’
GAPDH	F: 5’-GGAGCGAGATCCCTCCAAAAT-3’	60
	R: 5’-GGCTGTTGTCATACTTCTCATGG-3’