Table 3.
Primer sequence of target genes for RT-qPCR assay
| Organ | Genes | Sequence (5′->3′) |
|---|---|---|
| Gonadal fat pad | AD | F: TCAGGAAGAGGAGGAGGA |
| R: TCAGGAAGCACATCATACG | ||
| Glut1 | F: GCAGTTCGGCTATAACACTGG | |
| R: GCGGTGGTTCCATGTTTGATTG | ||
| Glut4 | F: CTGCTTCTGGCTCTCACA | |
| R: AGGACATTGGACGCTCTC | ||
| Glut8 | F: CCCTTCGTGACTGGCTTTG | |
| R: TGGGTAGGCGATTTCCGAGAT | ||
| Insulin receptor (IR) | F: CGATGGCTTCTCAGACGTG | |
| R: CAGCCCGCTTGTTGATGTTG | ||
| SREBP | F: TCATCAACAACCAAGACAGT | |
| R: CCAGAGAAGCAGAAGAGAAG | ||
| Liver | CPT1a | F: TGGCATCATCACTGGTGTGTT |
| R: GTCTAGGGTCCGATTGATCTTTG | ||
| G6PD | F: CACAGTGGACGACATCCGAAA | |
| R: GCAGGGCATTCATGTGGCT | ||
| Glucokinase | F: AGGAGGCCAGTGTAAAGATGT | |
| R: CTCCCAGGTCTAAGGAGAGAAA | ||
| Phosphofructokinase | F: TGCAGCCTACAATCTGCTCC | |
| R: GTCAAGTGTGCGTAGTTCTGA | ||
| 6-Phosphogluconate dehydrogenase | F: CCTCGACTCTGCTTCGTCTG | |
| R: AAGATCGCCTTGATGTGGGG | ||
| iNOS | F: GCACCGAGATTGGAGTTC | |
| R: TAGTTACACCGACACGAG | ||
| NF-kB | F: CATTCTGACCTTGCCTATCT | |
| R: TCTTACCTGTCTTGTCGTC | ||
| Housekeeping gene | β-Actin | F: TCCTTCCTGGGCATGGAG |
| R: AGGAGGAGCAATGATCTTGATCTT |