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Rapamycin通过诱导自噬增加放疗敏感性。A：A549细胞给予Rapamycin(100 nM)预处理24 h，放疗(4 Gy)后4 h收集细胞，采用电镜(10, 000×)检测自噬体。A：a：正常组；b：IR组；c：IR+RAPA组。B：采用Western blot检测Rapamycin(100 nM)预处理24 h，放疗(4 Gy)后4 h LC3蛋白质、p62蛋白质表达变化。C：QuanlityOne软件进行半定量分析，LC3蛋白质、p62蛋白质表达倍数变化。D：细胞克隆形成实验检测Rapamycin(100 nM)预处理24 h，放疗后A549细胞增殖情况。与N组相比，^*P < 0.05，与IR组相比，^△P < 0.05。

Rapamycin sensitized radiation by induced autophagy. A: A549 cells were incubated with 100 nmol/L rapamycin for 24 h before exposure to 4 Gy of irradiation, cells were extracted at 4 h after irradiation, autophagosomes were observed under transmission electron microscope (10, 000×). A: a: Control group; b: IR group; c: IR+RAPA group. B: A549 cells were incubated with 100 nmol/L rapamycin for 24 h before exposure to 4 Gy of irradiation, proteins were extracted at 4 h after irradiation, LC3Ⅱ/Ⅰ and p62 expression were detected by Western blot analysis. C: Semi-quantitated of LC3Ⅱ/Ⅰ and p62 expression using QuanlityOne software. D: The survival fraction of rapamycin-treated A549 cells for 24 h after exposure IR were analyzed by clonogenic assay. ^*P < 0.05 vs Control group, ^△P < 0.05 vs IR group.