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. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: Radiat Res. 2018 Mar 27;189(6):560–578. doi: 10.1667/RR14878.1

FIG. 10.

FIG. 10

Administration of LY364947 blocks all five TGF-β signaling pathways in Fancg−/− and Fanca−/− bone marrow stromal cell lines in vitro and in Fanca−/− bone marrow in vivo. Western blot analysis of each of the five TGF-β signaling pathways in each of six cell lines treated with 100 μM TGF-β with or without preincubation in 100 μM LY363947. Data are shown for marrow stromal cell lines of Fancg+/+, Fancg+/− and Fancg−/− (panel A) and Fanca+/+, Fanca+/−, and Fanca−/− (panel B). Panel C: Effects on TGF-β signaling by each of five pathways in freshly explanted marrow of in vivo treatment with LY364947 daily for 5 days after 30 Gy head and neck irradiation compared to irradiation alone, as described in Materials and Methods. (Densitometry shown in Supplementary Fig. S4; http://dx.doi.org/10.1667/RR14878.1.S1.)