FIG. 11.
TGF-β receptor antagonist drug LY364947 does not ameliorate distant marrow suppression by head and neck irradiation of Fanca−/− mice. Blocking of each of the five TGF-β signaling pathways by daily administration of LY364947 to Fanca−/− and control wild-type mice. Mice (n = 5/group) were studied individually. Groups of mice received single-fraction head and neck irradiation (30 Gy) according to Materials and Methods. At day 5 postirradiation, femur bone marrow was removed and quantitated for the number of CFU-GM colony-forming cells scored at day 7 in secondary semisolid media culture, as described in Materials and Methods. Compared to 0 Gy, for Fanca+/+ exposed to 30 Gy and to 30 Gy + LY364947, respectively, *P =0.0245 and *P =0.0297; and for Fanca−/− exposed to 30 Gy and to Gy + LY364947, respectively, *P = 0.0015 and *P = 0.0415.